Using liquid culture for the dauer assays has proven to be a little more tricky than expected. For whatever reason, we still haven’t been able to induce dauer even after drastically reducing the amount of food in the culture and doubling the ascaroside concentration found in the literature. Time to go back to the drawing board!
The leaving assays have been going well! Based on the data collected over the past couple weeks, we are going to hone in on a few of the sex reversal strains that looked promising. Over the final few weeks I’ll be working on collecting some more data and crunching numbers with Renee.
I can’t believe how this summer has flown by! In two short weeks we’ll be presenting our projects at a poster session- it seems unreal. I’m trying not to get sentimental just yet but as excited as I am to watch things come together over this final stretch, I can’t help wishing this experience wasn’t ending so soon.
Until next time! 🙂